Description:
Containing all the components needed to perform multiplex RT-qPCR swiftly and reliably in one reaction. The kit consists of 2X MMP One- Step RT-qPCR mastermix, containing chemically modified Hot Start Taq DNA polymerase, dUTP and UNG in enhancing buffer; 20X RTase/ RI enzyme mix, optimised for amplifying low copy RNA targets; and Just Water, our molecular biology grade water. The presence of thermolabile UNG and dUTP eliminates carryover contamination at room temperature. Unlike other UNG enzymes it is totally and irreversibly inactivated during thermocycling allowing post PCR analysis. This mix has been optimised for multiplex assays utilising dual labelled hydrolysis probes and is compatible with both standard and fast cycling conditions.
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